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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
26/02/2024 |
Data da última atualização: |
26/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DINIZ, G. de F. D.; COTA, L. V.; FIGUEIREDO, J. E. F.; LANA, U. G. de P.; MARINS, M. S.; SILVA, F. C.; SANTOS, V. L. dos; OLIVEIRA-PAIVA, C. A. |
Afiliação: |
GISELE DE FÁTIMA DIAS DINIZ; LUCIANO VIANA COTA, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MIKAELY SOUSA MARINS, UNIVERSIDADE FEDERAL DE LAVRAS; FELIPE CAMPOS SILVA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; VERA LÚCIA DOS SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS. |
Título: |
Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, v. 22, e1342, 2023. |
Idioma: |
Inglês |
Conteúdo: |
Fungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghum MenosFungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strat... Mostrar Tudo |
Palavras-Chave: |
Biocontrole; Fungo fitopatogênico. |
Thesagro: |
Fungo; Milho; Zea Mays. |
Thesaurus Nal: |
Bacillus (bacteria); Paenibacillus. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162254/1/Isolation-and-identification-of-broad-spectrum-antagonist-bacteria.pdf
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Marc: |
LEADER 02397naa a2200289 a 4500 001 2162254 005 2024-02-26 008 2023 bl uuuu u00u1 u #d 100 1 $aDINIZ, G. de F. D. 245 $aIsolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.$h[electronic resource] 260 $c2023 520 $aFungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghum 650 $aBacillus (bacteria) 650 $aPaenibacillus 650 $aFungo 650 $aMilho 650 $aZea Mays 653 $aBiocontrole 653 $aFungo fitopatogênico 700 1 $aCOTA, L. V. 700 1 $aFIGUEIREDO, J. E. F. 700 1 $aLANA, U. G. de P. 700 1 $aMARINS, M. S. 700 1 $aSILVA, F. C. 700 1 $aSANTOS, V. L. dos 700 1 $aOLIVEIRA-PAIVA, C. A. 773 $tRevista Brasileira de Milho e Sorgo$gv. 22, e1342, 2023.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
29/03/2006 |
Data da última atualização: |
30/11/2017 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
SILVA, H. T. da; FONSECA, J. R. |
Título: |
Banco ativo de germoplasma de feijão (Phaseolus vulgaris L.). |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 8., 2005, Goiânia. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2005. |
Volume: |
v. 2. |
Páginas: |
p. 1131-1135. |
Série: |
(Embrapa Arroz e Feijão. Documentos, 182). |
Idioma: |
Português |
Conteúdo: |
A introdução de germoplasma em câmara com ambiente controlado, a multiplicação e a disponibilização de banco de dados para utilização destas fontes importantes de genes, ampliam as opções de pesquisa nas mais diferentes linhas da ciência. |
Palavras-Chave: |
CONAFE; Multiplicação. |
Thesagro: |
Feijão; Gene; Germoplasma; Pesquisa; Phaseolus Vulgaris. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00992naa a2200241 a 4500 001 1214047 005 2017-11-30 008 2005 bl uuuu u00u1 u #d 100 1 $aSILVA, H. T. da 245 $aBanco ativo de germoplasma de feijão (Phaseolus vulgaris L.). 260 $c2005 300 $ap. 1131-1135. v. 2. 490 $a(Embrapa Arroz e Feijão. Documentos, 182).$vv. 2. 520 $aA introdução de germoplasma em câmara com ambiente controlado, a multiplicação e a disponibilização de banco de dados para utilização destas fontes importantes de genes, ampliam as opções de pesquisa nas mais diferentes linhas da ciência. 650 $aFeijão 650 $aGene 650 $aGermoplasma 650 $aPesquisa 650 $aPhaseolus Vulgaris 653 $aCONAFE 653 $aMultiplicação 700 1 $aFONSECA, J. R. 773 $tIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 8., 2005, Goiânia. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2005.
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